Search | Engineering

订阅投稿

首页工程期刊工程焦点工程成就工程前沿关于我们 English

资源类型

期刊论文 3

年份

2016 2

2009 1

关键词

社交标签系统，标签共现，频谱聚类，组相似度http://dx.doi.org/10.1631/FITEE.1500187， 1

检索范围：

排序：展示方式：

Construction and expression of hepatitis B virus vector encoding TC-tagged core protein

Yuanyuan LIN MD, Xiaoming CHENG MS, Yuhu SONG MD, Peiyuan LI MD, Ying CHANG MM, Jinjian YAO MD, Jusheng LIN MD, PhD, Li ZHOU PhD, Leiming XU PhD,

《医学前沿（英文）》 2009年第3卷第4期页码 396-402 doi: 10.1007/s11684-009-0056-z

摘要： Virus tagged with green fluorescent protein (GFP) contributes to the visualization and study of the virus in living cells. However, the hepatitis B virus (HBV) particle, which is a compact virion with limited internal space, cannot be incorporated with GFP tag as a large fragment. It was recently reported that protein genetically inserted with a smaller size tetracysteine (TC) tag could be specially labeled by a biarsenical fluorescent dye in living cells. In this study, we constructed a recombinant HBV vector encoding TC-tagged core protein for biarsenical labeling of HBV virion. TC tag was genetically inserted near the immunodominant c/e1 site of HBV core protein by mutagenesis. Western blot and enzyme-linked immunosorbent assay (ELISA) analysis showed that the TC-tagged core protein, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg) could be expressed in cells transfected with the recombinant HBV vector, which is similar to the cells transfected with wild-type HBV vector. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis showed that HBV virion formation was affected by the genetic insertion of TC tag into core protein in some degree, but cells transfected with the HBV vector could still produce HBV virions incorporated with TC-tagged core proteins. Taken together, the recombinant HBV vector can serve as a useful tool to produce HBV virions incorporated with TC-tagged core proteins to be fluorescently labeled by biarsencial dye for visualizing and studying HBV in living cells.

关键词： hepatitis B virus vector tetracysteine tag core protein

HTML PDF 收藏

基于共同共生群体相似度的社会标签聚类方法

Hui-zong LI,Xue-gang HU,Yao-jin LIN,Wei HE,Jian-han PAN

《信息与电子工程前沿（英文）》 2016年第17卷第2期页码 122-134 doi: 10.1631/FITEE.1500187

摘要：

社交标记系统广泛应用于Web 2.0。许多用户使用这些系统自由地创建，组织，管理和共享Internet资源。但是，社交标签系统产生的许多模糊且不受控制的标签不仅恶化了用户的体验，而且限制了资源的检索效率。标签聚类可以将具有相似语义的标签聚合在一起，并有助于缓解上述问题。在本文中，我们首先提出一种基于共生组相似度的通用方法，该方法利用用户，资源和标签之间的三元关系来度量标签之间的语义相关性。然后，我们提出了一种频谱聚类方法来解决标注数据的高维和稀疏性。实验结果表明，该方法是有效的。

关键词：社交标签系统，标签共现，频谱聚类，组相似度http://dx.doi.org/10.1631/FITEE.1500187，

HTML PDF 收藏

Annotation and validation of genes involved in photosynthesis and starch synthesis from a

Yang ZHANG,Xin CHEN,Haiyan WANG,Zhiqiang XIA,Peng LING,Wenquan WANG

《农业科学与工程前沿（英文）》 2016年第3卷第4期页码 308-320 doi: 10.15302/J-FASE-2016113

摘要： A full-length cDNA library from leaf and root tissues of cassava ( ) Arg7 and one accession of its wild ancestor W14 ( ssp. ) has been constructed. The library is comprised of four sub-libraries, containing 32640 recombinant clones, 6028 cDNA clones from their 5′ ends, and 128 clones from the 3′ ends were sequenced. In total, 5013 high-quality expressed sequence tags (ESTs) and 1259 unigenes were obtained. Of these, 746 unigenes were identified by their sequence homologies to ESTs from model plants, and 323 unigenes were mapped onto 114 different KEGG pathways. From these, 24 differentially expressed genes involved in starch metabolism and photosynthesis were identified and five of them were selected to compare their expression level between Arg7 and W14. Notably, Arg7 has a higher net photosynthesis rate in leaves, higher ribulose-1,5-bisphosphate carboxy-lase oxygenase activities in leaves, and higher AGPase activity in roots. This resource is the first EST collection from wild cassava and should be of value for gene discovery, genome annotation and studies of evolution.

关键词： Manihot esculenta expressed sequence tag unigene pathway expression pattern